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KMID : 1036420110040020073
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대한치의학회 영문학회지
2011 Volume.4 No. 2 p.73 ~ p.78
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A Study of a Sodium Bicarbonate Cotransporter Functionally Expressed in Xenopus laevis Oocytes
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Lee Tae-Hwan
Kim Sung-Beom
Park Kyung-Pyo
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Abstract
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Purpose: HCO3- is the most important ion to buffer the acidity of saliva. The transport of HCO3- is mediated by electrogenic Na+/HCO3- cotransporter 1 (NBCe1), which expressed in various tissues including salivary glands, kidney and pancreas, etc. This experiment was performed to investigate regulatory site of NBCe1involved in the pH regulation using various mutants of NBCe1.
Materials and Methods: Human parotid gland NBCe1 (hpNBCe1) and mutants by deletion of 1~285 bp and 1~1,035 bp were prepared. After microinjection of each cRNA to oocytes of Xenopus laevis, they were incubated for 2~3 days. The function of each protein was tested by electrophysiological method.
Results: When oocytes were exposed to the HCO3- buffered solution, 1~285 bp deleted mutant hpNBCe1 evoked a marked hyperpolarization ranging from -90 mV to -160 mV (average: -134 mV; n=12) compared to the full length of hpNBCe1. Although 1~1,035 bp deleted mutant hpNBCe1 was also expressed in the plasma membrane, but it did not show any changes of membrane potentials.
Conclusion: Our deletion mutant study demonstrated that 1~285 bp of the NBCe1 is the major domain to determine HCO3
- transport ratio.
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KEYWORD
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Sodium-Bicarbonate Cotransporter, Intracellular pH regulation, Xenopus laevis oocytes, Deleted mutant hpNBCe1
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